Correct answer is option (1), because ' X ' in the given diagram is ori while ' Y ' is rop. ' X ' which is ori is responsible for controlling the copy number of the linked DNA and ' Y which is rop codes for protein involved in the replication of plasmid. Options (2), (3) and (4) are incorrect as ' X ' and ' Y ' are not related to these functions.
The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ' X and ' Y genes: NEET 2024 Zoology,…
NEET UG 2024 — Zoology Biotechnology - Principles and Processes
The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ' X and ' Y genes: 
Held on 30 Apr 2024 · Verified 9 Jul 2026.
Options
- A
The gene ' X ' is responsible for controlling the copy number of the linked DNA and ' Y ' for protein involved in the replication of Plasmid.
- B
The gene ' X ' is for protein involved in replication of Plasmid and ' Y ' for resistance to antibiotics.
- C
Gene ' X ' is responsible for recognitions sites and ' Y 's responsible for antibiotic resistance.
- D
The gene ' X ' is responsible for resistance to antibiotics and ' Y ' for protein involved in the replication of Plasmid.
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Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes B. DNA ligase C. DNA mutase D. DNA recombinase E. DNA polymerase Choose the correct answer from the options given below:
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