Biotechnology - Principles and Processes PYQ

Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel. In the light of the above statements, choose the most appropriate answer from the options given below :

Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes B. DNA ligase C. DNA mutase D. DNA recombinase E. DNA polymerase Choose the correct answer from the options given below:

 In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

Polymerase chain reaction (PCR) amplifies DNA following the equation.

Identify the part of a bio-reactor which is used as a foam braker from the given figure. 

The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate. Given below are two statements about this method: Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies. Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies. In the light of the above statements, choose the most appropriate answer from the options given below:

Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:

Identify the incorrect statement related to gel electrophoresis.

What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism? A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism. B. It may get integrated into the genome of the recipient. C. It may multiply and be inherited along with the host DNA. D. The alien piece of DNA is not an integral part of chromosome. E. It shows ability to replicate. Choose the correct answer from the options given below:

Match List I with List II : $\begin{array}{|l|l|r|l|}\hline & \text{List-I} & & \text{List-II} \\\hline A. & \text{Lipase} & I. & \text{Peptide bond} \\\hline B. & \text{Nuclease} & II. & \text{Ester bond} \\\hline C. & \text{Protease} & III. & \text{Glycosidic bond} \\\hline D. & \text{Amylase} & IV. & \text{Phosphodiester bond} \\\hline\end{array}$ Choose the correct answer from the options given below :

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tet ${ }^R$ ) gene in the pBR322 cloning vector.

The "Ti plasmid" of Agrobacterium tumefaciens stands for

Following are the steps involved in the process of PCR. A. Annealing B. Amplification ( $\sim 1$ billion times) C. Denaturation D. Treatment with Taq polymerase and deoxynucleotides E. Extension Choose the correct sequence of steps of PCR from the options given below :

Which of the following are correct about EcoRI? A. Cut the DNA with blunt end B. Cut the DNA with sticky end C. Recognise a specific palindromic sequence D. Cut the DNA between the base G and A when encounters the DNA sequence 'GAATTC' E. Exonuclease Choose the correct answer from the options given below:

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

Which of the following statements is incorrect?

The following diagram showing restriction sites in $E$. coli cloning vector pBR322. Find the role of ' $X$ and ' $Y$ genes: 

Upon exposure to UV radation, DNA stained with ethidium bromide will show:

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

Which of the following can act as molecular scissors?

Thermostable DNA polymerase used in PCR was isolated from :

Which of the following statement is incorrect about Agrobacterium tumifaciens?

Match List-I with List-II.  Choose the correct answer from the options given below.

Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?