NEET UG 2020 — Biotechnology - Principles and Processes

In Recombinant $\mathrm{DNA}$ technology antibiotics are used: *Note: There was ambiguity in options in the original paper, hence the options are changed.

Choose the correct pair from the following.

Select the correct statement from the following:

Spooling is:

Identify the wrong statement with regard to restriction enzymes.

Match the following techniques or instruments with their usage: <table class="pyq-table"><tbody><tr><td>(a)</td><td>Bioreactor</td><td>(i)</td><td>Separation of DNA fragments</td></tr><tr><td>(b)</td><td>Electrophoresis</td><td>(ii)</td><td>Production of large quantities of products</td></tr><tr><td>(c)</td><td>$\mathrm{PCR}$</td><td>(iii)</td><td>Detection of pathogen, based on antigen-antibody reaction</td></tr><tr><td>(d)</td><td>$\mathrm{ELISA}$</td><td>(iv)</td><td>Amplification of nucleic acids</td></tr></tbody></table>Select the correct option from following:

First discovered restriction endonuclease that always cuts $\mathrm{DNA}$ molecule at a particular point by recognising a specific sequence of six base pairs is:

The specific palindromic sequence which is recognized by EcoRl is

In a mixture, $\mathrm{DNA}$ fragments are separated by:

In gel electrophoresis, separated DNA fragments can be visualized with the help of

The sequence that controls the copy number of the linked DNA in the vector, is termed

Match the organism with its use in biotechnology. <table class="pyq-table"><tbody><tr><td>(a)</td><td>Bacillus thuringiensis</td><td>(i)</td><td>Cloning vector</td></tr><tr><td>(b)</td><td>Thermus aquaticus</td><td>(ii)</td><td>Construction of first rDNA molecule</td></tr><tr><td>(c)</td><td>Agrobacterium tumefaciens</td><td>(iii)</td><td>DNA polymerase</td></tr><tr><td>(d)</td><td>Salmonella typhimurium</td><td>(iv)</td><td>Cry proteins</td></tr></tbody></table>