Zoology Biotechnology - Principles and Processes questions from NEET UG 2020.
First discovered restriction endonuclease that always cuts $\mathrm{DNA}$ molecule at a particular point by recognising a specific sequence of six base pairs is:
In Recombinant $\mathrm{DNA}$ technology antibiotics are used: *Note: There was ambiguity in options in the original paper, hence the options are changed.
Select the correct statement from the following:
Spooling is:
Identify the wrong statement with regard to restriction enzymes.
In gel electrophoresis, separated DNA fragments can be visualized with the help of
Match the following techniques or instruments with their usage: <table class="pyq-table"><tbody><tr><td>(a)</td><td>Bioreactor</td><td>(i)</td><td>Separation of DNA fragments</td></tr><tr><td>(b)</td><td>Electrophoresis</td><td>(ii)</td><td>Production of large quantities of products</td></tr><tr><td>(c)</td><td>$\mathrm{PCR}$</td><td>(iii)</td><td>Detection of pathogen, based on antigen-antibody reaction</td></tr><tr><td>(d)</td><td>$\mathrm{ELISA}$</td><td>(iv)</td><td>Amplification of nucleic acids</td></tr></tbody></table>Select the correct option from following:
Match the organism with its use in biotechnology. <table class="pyq-table"><tbody><tr><td>(a)</td><td>Bacillus thuringiensis</td><td>(i)</td><td>Cloning vector</td></tr><tr><td>(b)</td><td>Thermus aquaticus</td><td>(ii)</td><td>Construction of first rDNA molecule</td></tr><tr><td>(c)</td><td>Agrobacterium tumefaciens</td><td>(iii)</td><td>DNA polymerase</td></tr><tr><td>(d)</td><td>Salmonella typhimurium</td><td>(iv)</td><td>Cry proteins</td></tr></tbody></table>
Choose the correct pair from the following.
The specific palindromic sequence which is recognized by EcoRl is
In a mixture, $\mathrm{DNA}$ fragments are separated by:
The sequence that controls the copy number of the linked DNA in the vector, is termed