NEET UG 2022 — Biotechnology - Principles and Processes

Separation of DNA, fragments is done by a technique known as :

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Polymerase chain reaction is used in DNA amplification Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation In the light of the above statements, choose the correct answer from the options given below :

Refer to the following statements for agarose-gel electrophoresis : (a) Agarose is a natural polymer obtained from sea-weed. (b) The separation of DNA molecules in agarosegel electrophoresis depends on the size of DNA. (c) The DNA migrates from negatively-charged electrode to the positively-charged electrode (d) The DNA migrates from positively-charged electrode to the negatively-charged electrode. Choose the most appropriate answer from the options given below :

Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A) : When a particular restriction enzyme cuts strand of DNA, overhanging stretches or sticky ends are formed. Reason (R) : Some restriction enzymes cut the strand of DNA a little away from the centre of palindromic site. In the light of the above statements, choose the correct answer from the options given below :

The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct pair of options from the following:

Which one of the following statement is not true regarding gel electrophoresis technique?

Which of the following methods is not commonly used for introducing foreign DNA into the plant cell?

Given below are two statements: Statement $I$: Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence. Statement $\mathrm{II}$: Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site. In the light of the above statements, choose the most appropriate answer from the options given below:

Pathogenic bacteria gain resistance to antibiotics due to changes in their :

Match List-I with List-II :  Choose the correct answer from the options given below :

Which of the following is not a desirable feature of a cloning vector?

In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?