NEET UG 2024 — Biotechnology - Principles and Processes

The following diagram showing restriction sites in $E$. coli cloning vector pBR322. Find the role of ' $X$ and ' $Y$ genes: 

Which of the following statements is incorrect?

Match List I with List II : $\begin{array}{|l|l|r|l|}\hline & \text{List-I} & & \text{List-II} \\\hline A. & \text{Lipase} & I. & \text{Peptide bond} \\\hline B. & \text{Nuclease} & II. & \text{Ester bond} \\\hline C. & \text{Protease} & III. & \text{Glycosidic bond} \\\hline D. & \text{Amylase} & IV. & \text{Phosphodiester bond} \\\hline\end{array}$ Choose the correct answer from the options given below :

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tet ${ }^R$ ) gene in the pBR322 cloning vector.

The "Ti plasmid" of Agrobacterium tumefaciens stands for

Following are the steps involved in the process of PCR. A. Annealing B. Amplification ( $\sim 1$ billion times) C. Denaturation D. Treatment with Taq polymerase and deoxynucleotides E. Extension Choose the correct sequence of steps of PCR from the options given below :

Which of the following are correct about EcoRI? A. Cut the DNA with blunt end B. Cut the DNA with sticky end C. Recognise a specific palindromic sequence D. Cut the DNA between the base G and A when encounters the DNA sequence 'GAATTC' E. Exonuclease Choose the correct answer from the options given below:

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism? A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism. B. It may get integrated into the genome of the recipient. C. It may multiply and be inherited along with the host DNA. D. The alien piece of DNA is not an integral part of chromosome. E. It shows ability to replicate. Choose the correct answer from the options given below:

Identify the incorrect statement related to gel electrophoresis.

Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with: