Option (2) is the answer because, In pBR322, the restriction site for BamHI is present in tet R gene. Therefore, ligation of a foreign DNA will results in loss of tetracycline resistance and recombinants will be tetracycline sensitive. Option (1) is not the answer because, In pBR322, the restriction site of EcoR I is present outside the region of ampR gene and tet R gene. Option (3) and (4) are not the answers because, in pBR322, the restriction sites for PstI and PvuI are present in ampR gene. So, ligation of foreign DNA will result in loss of ampicillin resistance and recombinants will be ampicillin sensitive.
Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?
NEET UG 2023 — Zoology Biotechnology - Principles and Processes
2023mcqeasy
Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?
Official previous-year question
Held on 30 Apr 2023 · Verified 9 Jul 2026.
Options
- A
EcoR I
- B
BamH I
- C
Pst I
- D
Pvu I
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