Biotechnology and Its Applications PYQ

Which of the following genetically engineered organisms was used by Eli Lilly to prepare human insulin?

Silencing of specific mRNA is possible via RNAi because of

Given below are two statements : Statement I : Transfer RNAs and ribosomal RNA do not interact with mRNA. Statement II : RNA interference (RNAi) takes place in all eukaryotic organisms as a method of cellular defence. In the light of the above statements, choose the most appropriate answer from the options given below :

The $B t$ toxin in genetically engineered $B t$ cotton kills the pest by:

Match List I with List II :  Choose the correct answer form the options given below:

Given below are two statements : Statement I : RNA interference takes place in all Eukaryotic organisms as method of cellular defense. Statement II : RNAi involves the silencing of a specific mRNA due to a complementary single-stranded RNA molecule that binds and prevents translation of mRNA In the light of the above statements, choose the correct answer from the options given below.

Given below are two statements: Statement I : Bt toxins are insect group specific and coded by a gene cry IAc. Statement II : Bt toxin exists as inactive protoxin in B. thuringiensis. However, after ingestion by the insect the inactive protoxin gets converted into active form due to acidic pH of the insect gut. In the light of the above statements, choose the correct answer from the options given below:

Match List-I with List-II : \begin{array}{llll} List-I & & List-II \\A.Genetically engineered Human Insulin & I. & Gene therapy \\B. GM Cotton & II.& E. coli \\C. ADA Deficiency & III.& Antigen-antibody interaction \\D. ELISA & IV. & Bacillus thuringiensis\end{array} Choose the correct answer from the options given below:

Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment?

Select incorrect statement, regarding chemical structure of insulin.

Match List - I with List - II.  Choose the correct answer from the options given below :

Statements related to human Insulin are given below: Which statement(s) is/are correct about genetically engineered Insulin? (a) Pro-hormone insulin contain extra stretch of C-peptide. (b) A-peptide and B-peptide chains of insulin were produced separately in E. coli, extracted and combined by creating disulphide bond between them. (c) Insulin used for treating Diabetes was extracted from cattle and pigs. (d) Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone. (e) Some patients develop allergic reactions to the foreign insulin. Choose the most appropriate answer from the options given below.

Milk of transgenic 'Cow Rosie' was nutritionally more balanced product for human babies than natural cow milk because it contained:

In gene therapy of adenosine deaminase (ADA) deficiency, the patient requires a periodic infusion of genetically engineered lymphocytes because:

Breedings crops with higher levels of vitamins and minerals or higher proteins and healthier fats is called:

Transposons can be used during which one of the following?

For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?

The Adenosine deaminase deficiency results into:

With regard to insulin choose correct options. (a) C-peptide is not present in mature insulin. (b) The insulin produced by rDNA technology has C-peptide. (c) The pro-insulin has C-peptide. (d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges.

When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as:

Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because:

Which of the following statements is not correct?

The laws and rules to prevent unauthorised exploitation of bioresources are termed as:

$\mathrm{RNA}$ interference is used for which of the following purposes in the field of biotechnology?