During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Held on 30 Apr 2021 · Verified 9 Jul 2026.
Annealing
Extension
Denaturation
Ligation
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The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate. Given below are two statements about this method: Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies. Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies. In the light of the above statements, choose the most appropriate answer from the options given below:
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