DNA is highly polarised due to phosphate backbone. This makes it highly soluble in water. Generally, 0.3 M Sodium Acetate is used which neutralizes the negative charge on the PO3- groups on the nucleic acids, making the molecule far less hydrophilic, and therefore much less soluble in water. Then, three volumes of (at least) 95% ethanol is added that removes the hydration shell of H2O molecules around the phosphate.
DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:
NEET UG 2019 — Zoology Biotechnology - Principles and Processes
DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:
Held on 30 Apr 2019 · Verified 9 Jul 2026.
Options
- A
Isopropanol
- B
Chilled ethanol
- C
Methanol at room temperature
- D
Chilled chloroform
Did you get this right?
Sign in to track your attempts and accuracy.
Your note
Sign in to keep a private note on this question. Nothing you write is ever public.
NEET UG Biotechnology - Principles and Processes in other years
More NEET UG Biotechnology - Principles and Processes questions
Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes B. DNA ligase C. DNA mutase D. DNA recombinase E. DNA polymerase Choose the correct answer from the options given below:
The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate. Given below are two statements about this method: Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies. Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies. In the light of the above statements, choose the most appropriate answer from the options given below:
Identify the part of a bio-reactor which is used as a foam braker from the given figure. 
Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel. In the light of the above statements, choose the most appropriate answer from the options given below :
 In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
Other Zoology topics for NEET UG
Keep practicing Biotechnology - Principles and Processes
Work through every NEET UG Biotechnology - Principles and Processes PYQ, year by year.