To facilitate cloning into a vector, the vector requires a selectable marker, which helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.
A selectable marker is used to
Held on 30 Apr 2019 · Verified 9 Jul 2026.
help in eliminating the non-transformants, so that the transformants can be regenerated
identify the gene for a desired trait in an alien organism
select a suitable vector for transformation in a specific crop
mark a gene on a chromosome for isolation using restriction enzyme
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The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate. Given below are two statements about this method: Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies. Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies. In the light of the above statements, choose the most appropriate answer from the options given below:
 In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
Identify the part of a bio-reactor which is used as a foam braker from the given figure. 
Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes B. DNA ligase C. DNA mutase D. DNA recombinase E. DNA polymerase Choose the correct answer from the options given below:
Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel. In the light of the above statements, choose the most appropriate answer from the options given below :
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