There are three steps in polymerase chain reaction namely. (i) Denaturation-at 94∘C for DNA strand separation. (ii) Annealing-binding of primer. (iii) Extension in presence of DNA polymerase which is heat stable.
NEET UG 2012 — Zoology Biotechnology - Principles and Processes
2012mcqeasy
The figure below shows three steps (I, II, III) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? 
Official previous-year question
Held on 30 Apr 2012 · Verified 9 Jul 2026.
Options
- A
B-Denaturation at a temperature of about 98∘C separating the two DNA strands
- B
A-Denaturation at a temperature of about 50∘C
- C
C-Extension in the presence of heat stable DNA polymerase
- D
A-Annealing with two sets of primers
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