DNA ligase is used to seal the nicks that remain in recombinant DNA molecule. Infact DNA ligase joins together the neighbouring nucleotides flanking a discontinuity in a DNA strand by forming a phosphodiester bond. A recombinant DNA molecule is a vector into which the desired DNA fragment has been inserted to enable its cloning in an appropriate host. Endonucleases are the enzymes that produce internal cuts called cleavage in DNA molecules. A class of endonucleases cleaves DNA only within or near those sites, which has specific base sequences, such endonucleases are known as restriction endonucleases. Exonucleases are enzymes that remove one or more nucleotide from the free ends. DNA polymerase is the enzyme, which cause polymerization of nucleotides during DNA replication.
The linking of antibiotic resistance gene with the plasmid vector became possible with
NEET UG 2008 — Zoology Biotechnology - Principles and Processes
The linking of antibiotic resistance gene with the plasmid vector became possible with
Held on 30 Apr 2008 · Verified 9 Jul 2026.
Options
- A
DNA ligase
- B
endonucleases
- C
DNA polymerase
- D
exonucleases
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Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel. In the light of the above statements, choose the most appropriate answer from the options given below :
Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes B. DNA ligase C. DNA mutase D. DNA recombinase E. DNA polymerase Choose the correct answer from the options given below:
The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate. Given below are two statements about this method: Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies. Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies. In the light of the above statements, choose the most appropriate answer from the options given below:
Identify the part of a bio-reactor which is used as a foam braker from the given figure. 
 In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
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