Okazaki fragments in DNA are linked up by the enzyme DNA ligase after replacing the RNA primers with deoxyribonucleotides after replacing the RNA primers with deoxyribonucleotides solution of the Okazaki fragments in DNA chain growth. Replication occurs always in 5'-3' direction. However, Okazaki fragments are synthesized on 3'-5' DNA template, join to form lagging strand which grows in 3′−5′ direction.