When genomic DNA extracted from any tissue of a plant or animal species is digested with a restriction enzyme, it is cleaved into segments. The segments of different sizes can be separated through gel electrophoresis. Gel electrophoresis involves movement of fragments or molecules from a well created on one edge of the gel. Endonucleases are enzymes that produce internal cuts called cleavage in DNA molecules. A class of endonucleases cleaves DNA only within or near those sites, which have specific base sequences, such endonucleases are known as restriction endonucleases. The selected vector is cut open with a restriction enzyme that has a unique target site located in the sequence where the DNA insert should be integrated to facilitate the selection of recombinant DNA.
Gel electrophoresis is used for
NEET UG 2008 — Zoology Biotechnology - Principles and Processes
Gel electrophoresis is used for
Held on 30 Apr 2008 · Verified 9 Jul 2026.
Options
- A
cutting of DNA into fragments
- B
separation of DNA fragments according to their size
- C
construction of recombinant DNA by joining with cloning vectors
- D
isolation of DNA molecule
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