The simplest living organisms could not have originated spontaneously from non-living matter. Microbes were killed by heating the meat and the sealed vessel formed a closed system wherein the new microbes could not come in contact with the nutrient medium and hence no spoilage of meat. Related Theory Pasteur performed experiments in which he took sterilized yeast and sugar solution in a long necked, then he bent the neck of the flask like a neck of swan. After one month he observed that no life appeared in flask solution because the curved flask neck acts as a filter. He later on broken down the neck and observed the solution. He found that many micro-organisms were originated in solution.