Based on the hypothesis proposed by Oparin and Haldane, S.L. Miller provided experimental evidence of the chemical evolution in 1953 in a laboratory set-up. S.L. Miller, an American Scientist, created similar conditions as described by Oparin and Haldane on a laboratory scale. He sealed the gases CH_{4, }H₂, NH3, and water vapor at 800 oC in a closed flask and made an arrangement of two electrodes in this flask. The electrodes created the electric discharge which provided the energy for the chemical reactions between these gases. A condenser was attached to condense the gaseous compounds formed in the flask into the liquid form. After some time (days), he observed the formation of amino acids like glycine, alanine, and aspartic acid, in the flask from inorganic compounds.