Biotechnology - Principles and Processes PYQ — Page 3

Select the correct statement from the following:

Identify the wrong statement with regard to restriction enzymes.

First discovered restriction endonuclease that always cuts $\mathrm{DNA}$ molecule at a particular point by recognising a specific sequence of six base pairs is:

In gel electrophoresis, separated DNA fragments can be visualized with the help of

The sequence that controls the copy number of the linked DNA in the vector, is termed

The specific palindromic sequence which is recognized by EcoRl is

Choose the correct pair from the following.

Match the organism with its use in biotechnology. <table class="pyq-table"><tbody><tr><td>(a)</td><td>Bacillus thuringiensis</td><td>(i)</td><td>Cloning vector</td></tr><tr><td>(b)</td><td>Thermus aquaticus</td><td>(ii)</td><td>Construction of first rDNA molecule</td></tr><tr><td>(c)</td><td>Agrobacterium tumefaciens</td><td>(iii)</td><td>DNA polymerase</td></tr><tr><td>(d)</td><td>Salmonella typhimurium</td><td>(iv)</td><td>Cry proteins</td></tr></tbody></table>

Match the following techniques or instruments with their usage: <table class="pyq-table"><tbody><tr><td>(a)</td><td>Bioreactor</td><td>(i)</td><td>Separation of DNA fragments</td></tr><tr><td>(b)</td><td>Electrophoresis</td><td>(ii)</td><td>Production of large quantities of products</td></tr><tr><td>(c)</td><td>$\mathrm{PCR}$</td><td>(iii)</td><td>Detection of pathogen, based on antigen-antibody reaction</td></tr><tr><td>(d)</td><td>$\mathrm{ELISA}$</td><td>(iv)</td><td>Amplification of nucleic acids</td></tr></tbody></table>Select the correct option from following:

In a mixture, $\mathrm{DNA}$ fragments are separated by:

DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:

Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements. 1. DNA is negatively charged molecule and so it is loaded on gel towards the anode terminal. 2. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA. 3. Smaller the size of DNA fragment larger is the distance it travels through it. 4. Pure DNA can be visualised directly by exposing UV-radiation. Select the correct option from the following

An enzyme catalysing the removal of nucleotides from ends of DNA is

The two antibiotic resistant genes on vector pBR322 are for

A selectable marker is used to

Match the following enzymes with their functions  Select the correct option from the following

Following statements describe the characteristics of restriction endonucleases. Identify the incorrect statement.

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

The correct order of steps in Polymerase Chain Reaction (PCR) is

The DNA fragments separated on an agarose gel can be visualised after staining with:

The process of separation and purification of expressed protein before marketing is called

What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?

A gene whose expression helps to identify transformed cell is known as

The Taq polymerase enzyme is obtained from: