When 3-phosphoglyceraldehyde is converted into 1,3 diphosphoglyceric acid, two electrons and two protons are released which are utilised to convert NAD+to NADH. NAD++2H++2e−→NADH+H+ Related Theory Glycolysis is a partial breakdown of a sixcarbon glucose molecule into two, threecarbon molecules of pyruvate, 2NADH,2H+, and 2 net ATP as a result of substrate-level phosphorylation. Glycolysis occurs in the cytoplasm of the cell.