In all myosin, the head domain is a specialized ATPase that is able to couple the hydrolysis of ATP with motion. Related Theory The contractile protein of skeletal muscle involving ATPase activity is myosin. Myosin comprise a family of ATP-dependent motor proteins and are best known for their role in muscle contraction and their involvement in a wide range of other eukaryotic motility processes. They are responsible for actinbased motility.